ABSTRACT
PURPOSE: The β3-adrenergic receptor (ADRB3) is expressed in visceral adipose tissue and has been speculated to contribute to lipolysis, energy metabolism, and regulation of the metabolic rate. In this study, we aimed to investigate the association of polymorphism of the ADRB3 gene with the sex of children with obesity and related pathologies. METHODS: ADRB3 gene trp64arg genotyping was conducted in 441 children aged 6–18 years. Among these subjects, 264 were obese (103 boys; 161 girls) and 179 were of normal weight (81 boys; 98 girls). In the obese group, fasting lipids, glucose and insulin levels, and blood pressure were measured. Metabolic syndrome (MS) was defined according to the modified World Health Organization criteria adapted for children. RESULTS: The frequency of trp64arg genotype was similar in obese and normal weight children. In obese children, serum lipid, glucose, and insulin levels; homeostasis model assessment of insulin resistance (HOMA-IR) scores; and MS were not different between arg allele carriers (trp64arg) and noncarriers (trp64trp). In 264 obese children, genetic analysis results revealed that the arg allele carriers were significantly higher in girls than in boys (p=0.001). In the normal weight group, no statistically significant difference was found between genotypes of boys and girls (p=0.771). CONCLUSION: Trp64arg polymorphism of the ADRB3 gene was not associated with obesity and MS in Turkish children and adolescents. Although no relationships were observed between the genotypes and lipids, glucose/insulin levels, or HOMA-IR, the presence of trp64arg variant was frequent in obese girls, which can lead to weight gain as well as difficulty in losing weight in women.
Subject(s)
Adolescent , Child , Female , Humans , Alleles , Blood Pressure , Energy Metabolism , Fasting , Genotype , Glucose , Homeostasis , Insulin , Insulin Resistance , Intra-Abdominal Fat , Lipolysis , Obesity , Pathology , Weight Gain , World Health OrganizationABSTRACT
Objective: To evaluate the lipid profile in Multibacillary and Paucibacillary leprosy subjects and compare them with age and sex matched healthy control subjects
Materials and Methods: This observational study was performed after approval from BASR, University of Karachi in the Department of Biochemistry, University of Karachi, from December 2014 to November, 2015. Present study was conducted in 42 newly diagnosed leprosy patients of both sexes and all ages were included in this study. The diagnosis were on clinical ground and bacterial examination by slit skin smear test, and are classified in two groups, Paucibacillary [PB] and Multibacillary [MB], based on the WHO guide lines. 1-5 skin lesions were regarded as PB with no acid fast rods on the smear and skin lesions more than 5 were regarded as MB. A positive bacterial index classifies the patient as MB, regardless of the number of skin lesions with bacteria visible on a smear
Results: A total of 30 control subjects and 42 leprosy patients among 24 Multibacillary and 18 Paucibacillary leprosy were recruited for this study. Biophysical parameters in Multibacillary and Paucibacillary subjects were completely non significant when compared with control group.In biochemical parameters among Multibacillary and Paucibacillary leprosy cases, all the lipid fractions total cholesterol, triglycerides and LDL -cholesterol were significantly decreased [p<0.05] but HDL -cholesterol significantly increased [p<0.05] in both Multibacillary and Paucibacillary leprosy groups when compared with control group
Conclusion: This study showed that, all the lipid fractions except HDL cholesterol were decreased significantly [p<0.05], where as HDL Cholesterol was increased significantly [p<0.05] in both Multibacillary and Paucibacillary leprosy groups when compared with control group
ABSTRACT
It is an open-label comparative study, conducted at the Department of Pharmacology and Therapeutics, Basic Medical Sciences Institute, Jinnah Postgraduate Medical Centre, Karachi. The study comprised of two drugs of different groups; total eighty adult patients were enrolled [44 female and 36 male] from the department of psychiatry. Enrolled patients received trazodone 50 mg daily which was gradually increased to 150 mg in divided doses and paroxetine 20 mg daily for a period of 90 days. The results revealed that paroxetine is more effective and has less adverse effects than the trazodone. There was significant improvement in symptoms of sexual disturbance and weight loss of major depressive disorder patients receiving paroxetine